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CapillaryTrack

by Frederic Marion-Poll

Build kymographs to monitor the evolution of the level of a liquid in capillary tubes

Publication Id
ICY-V5N6C4
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Documentation

This plugin is meant to analyze images where 20 capillaries filled with a liquid can be consumed by flies. A webcam placed over this box takes 1 image per min. We want to follow the liquid level across time. The idea is to place ROI line over each capillary and to follow the profile of the pixels over time using kymographs. The plugin allows to:

- read a stack of jpg files or an AVI in "virtual" mode (images read are "buffered" in a separates thread)

- place an array of line ROIs over the image

- generate an array of kymographs corresponding to the profile of each line across time

- filter the kymographs

- detect a path from each kymograph (using an adjustable threshold) over the raw data or the filtered data and export the results to Excel.