Input: 3D or 4D sequence (1 color channel)
Output: filtered sequence
Iterations: number of iteration for the computation. Higher value gives more filtered data.
Sigma: size of 1st Gaussian kernel (local filter)
Ro: size of 2nd Gaussian kernel (integration scale for the structure tensor); higher values can generate false structures around membrane
Z/X ratio: ratio between the depth (Z) resolution and the XY resolution
K PM: threshold for Perona Malik filter (advice: keep 5) higher value gives more isotropic effects
Threshold: important parameter which select the 1D and 2D behavior of the filter. It's the threshold for the fuzzy selection function.
4D stack: select this option only if you want to process a 4D stack with multi thread option
No threads: number of thread. one thread is applied on one time-stack. May create out of memory errors.