This plugin allows the statistical analysis of the spatial distributions of molecules in one or several field of views (ROIs).
It is based on the Ripley's K function which counts the number of pairs of points that are closer than a parameter r. Comparing the values of the K function to its critical quantiles under spatial randomness then allows to assess statistically the presence of clusters (K(r)>quantile at level (1-alpha)) or dispersion (K(r)< quantile at level alpha) (see scheme below)
How to use the plugin in detail:
The methodology is detailed in the article
-T. Lagache, G. Lang, N. Sauvonnet and J.-C. Olivo-Marin Analysis of the spatial organization of molecules with robust statistics. PLoS ONE (Accepted, 2013).
A PPT presentation of the article is shown hereafter: