Need help or advice ? Come to the Icy club ! - Every Wenesday morning from 9h30 to 12h30 - Francois Jacob Building - Main hall - Pasteur
Register

User reviews

(1)
5 star
1
4 star
0
3 star
0
2 star
0
1 star
0
average rating: 5


Please log-in to post a review
Fabrice de Chaumont
14 Nov 2013 17:16
Very well documented ! Good idea to put the full presentation !
It's impressive to see how impossible it is to distinguish clusters by eye. Good to have this quantification !

Spatial Analysis

by Thibault Lagache

This plugin uses the Ripley's K function to analyze robustly the spatial organization of molecules.

Publication Id
ICY-P2C9P5
See technical details
View complete changelog

Documentation

Introduction:

This plugin allows the statistical analysis of the spatial distributions of molecules in one or several field of views (ROIs).

It is based on the Ripley's K function which counts the number of pairs of points that are closer than a parameter r. Comparing the values of the K function to its critical quantiles under spatial randomness then allows to assess statistically the presence of clusters (K(r)>quantile at level (1-alpha)) or dispersion (K(r)< quantile at level alpha) (see scheme below)

How to use the plugin in detail:

The methodology is detailed in the article

-T. Lagache, G. Lang, N. Sauvonnet and J.-C. Olivo-Marin Analysis of the spatial organization of molecules with robust statistics. PLoS ONE (Accepted, 2013).

A PPT presentation of the article is shown hereafter: