TED is a plugin for ICY detecting ellipsoidal shapes in 2D and 3D images. It outputs a list of ROI.
||Expected radius range of the cells to detect.|
|Target Brightness||Indicates if the the cells are darker than the background (Dark) or the opposite (Bright).|
|Threshold||Controls the segmentation step. A small value leads to the detection of less contrasted objects.|
|Optimize Descriptors||Indicates if the descriptors of the detected cells have to be optimized.|
|Maximum number of iterations||Maximum number of iterations during the optimization process.|
Remark: The preview checkbox allows the user to see the result of the segmentation step. It could be a good idea to use that in order to play with the threshold parameter, before starting the whole process.