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Widefield Fluorescence Microscope PSF

by Praveen Pankajakshan

This plugin generates the point-spread function (PSF) for a Widefield Fluorescence Microscope using a scalar diffraction-limited model (Stokseth). It can generate PSF with spherical aberration if there is refractive index mismatch between objective immersion and specimen mounting media.


[1] P. A. Stokseth (1969). `Properties of a defocused optical system'. J. Opt. Soc. Am. A 59:1314-1321.

[2] P. Pankajakshan (2009). Blind Deconvolution for Confocal Laser Scanning Microscopy. Ph.D. thesis, Universite de Nice Sophia-Antipolis.

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