Short DescriptionThis plugin measures the percentage of elongated spots in a sequence.
This plugin can be used to quantify the percentage of "elongated" (not round) objects in an image.
1-Objects (spots) must be first segmented with Spot detector.
2- User must specify a shape descriptor of the spots:
Morton criterion=Area/(Length of the principal axis)4
3-User must also specify a threshold (T)
4-The plugin then computes the percentage of "elongated" objects (spots) whose shape criterion (Circularity or Morton) is below T (Circularity criterion T=1 for perfectly round objects and T tends to 0 for very elongated objects)
5-These quantitative informations can be exported in an Xls file