Hi Masoud,

We will try to replicate your different problems using Micro-Manager with Multi-D acquisition and multi position and get back to you after our discovers. About the automatic stitching problem : you can disable it, just select “separate files” for grouping mode :

So it will allow you to open independent image, otherwise grouping will automatically try to stitch all the position acquired to build a big image out of it.

Best,

– Stephane