Hi Jacob,

Normally Icy should automatically stitch the image when you select “group files” in the loading dialog :

Still that will be a very basic stitching without considering overlap area, and it works only if all the images contained in the multi-series file have all the exact same size. For instance in my case above the multi-series file contains a preview image with a different size so stitching doesn’t operate.

If you want an advanced stitching tool you may try this :

https://imagej.net/Image_Stitching

As i don’t think Icy contains that kind of tool.

Best,

– Stephane