Good morning, I think I have the same problem and question. After applying “Spot Detector” and “Colocalization Studio”, I can’t find the colocalization files or images with ROI or set parameters of Roi, that the CoLocS plugin allows to export. When I name the excel file and I choose the file saving path, the plugin only exports the excel file with the three possible analyzes: distance analysis, parametric and soda Ripley’s. I hope in your help to use this interesting analysis program. Thank you, Chiara Elia