Ok now i’m starting to see what you tried to do 😉 Indeed by default channel information is always set to ALL (-1 internally) and there is no way to change that behavior unfortunately. I guess you know you can change default color of ROI, and also change channel information globally (just doing multi selection of ROI then changing the channel index information). I don’t have better to propose but i admit that is not really convenient for what you’re trying to do.

Best,

– Stephane