Hi Audrey,

Unfortunately that is a limitation of the Spot Detector plugin, it’s not really designed to detect spots with important difference in size.

But you can use the protocol to adapt this and make several spot detection and combine the results. You can see an example with the protocol “Spot Detector scale 2 and scale 3” which is available online. Just type its name in the top search bar of Icy, you should be able to access it 🙂

Hope that helps !

– Stephane