Hello Audrey,
I had the same problem few times ago. What I did to solve this problem was to use different parameters of spot detector on the same image. Found 2 or 3 Spot Detector parameters which could englobe your whole spots (which are also separeted as in your picture) and use the Boolean operation in “Protocol”. The result will be to have different size of spots and for them which are superposed (because they will be detected with both of 2 parameters) they will be merge in one. Hope I help you atleast a bit, good luck for the rest =)
Victor
