Need help or advice ? Come to the Icy club ! - Every Wenesday morning from 9h30 to 12h30 - Francois Jacob Building - Main hall - Pasteur
Register

User reviews

This plugin is not rated yet


Please log-in to post a review

MicroscPSF

by Jizhou LI

Implementing a (very) fast microscopic PSF (specifically, the Gibson-Lanni model) generation.

Publication Id
ICY-W9C5P4
See technical details
View complete changelog

Documentation

This plugin implements a fast microscopy PSF generation method. The model is selected as the Gibson-Lanni model, which is particularly useful since it involves explicitly design and experimental physical parameters. 

References:

  • [1] J. Li, F. Xue and T. Blu, “Fast and Accurate Three-Dimensional Point Spread Function Computation for Fluorescence Microscopy”, J. Opt. Soc. Am. A, vol. 34, no. 6, pp. 1029-1034, 2017. 
  • [2] S. F. Gibson and F. Lanni, “Experimental test of an analytical model of aberration in an oil-immersion objective lens used in three-dimensional light microscopy”, J. Opt. Soc. Am. A, vol. 9, no. 1, pp. 154-166, 1992.
Key points:
 
  • We express the integral in this model as a linear combination of rescaled Bessel functions, providing an integral-free way for the calculation.
  • Experiments demonstrate that the proposed approach results in significantly smaller computational time compared with the quadrature approach.
  • This approach can also be extended to other microscopy PSF models.

Related:

Screenshot:

GUI