Kymograph Tracker

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  • Claudia Carcamo

    I would like to use Kymograph Tracker to extract the trajectories of particles in a kymograph that I already have generated.

    I am not starting with an image stack, but rather a kymograph.  This is because I collect data as confocal point scanning, and visualize the data as a kymograph.

    Kymograph Tracker seems require that you start the protocol by specifying a region of interest (in an image stack) and from there it generates a kymograph that can be used to extract trajectories.

    I would like to skip this first part of the protocol, and provide a kymograph.

    Is this possible, and if so, what is the procedure to have the software accept my kymograph such that I can use the trace extraction features.

    Thank you.

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