I’ve just tried this and it worked! The ROI statistics from the protocol and when manually obtained are identical. (In case you are wondeirng, the values match with the manually obtained ROI statistics from the screenshot above).
Apologies for the late response – I tried to sent a reply a few weeks ago but it looks like it never went through. I have tried several times to reply now but each time the message doesn’t appear on the thread. I’ve attached some screenshots of my protocol with an example of a processed image. I will try sending one screenshot at a time.
Part 1:
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