this protocols allow to calculate internal leaf area. first of all, the destination excel file need to be create in order to save results, than the last protocol block (workbook to file) need to be adjusted with the right destination file.
In order to provide the correct mesure unit you have to calculate the value of a single pixel (X size or Y size); than, in result spredsheet, you can calculate area with this formula INTERNAL PIXEL* X pixel size* Y pixel size.
The result will be sorted by Y posizion value
It’s possible to add, remove or change the result showed clicking the botton in the block called -ROI Statistics-