Active contour – limit object size/intensity

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    deborakeller0@gmail.com May 29, 2019 at 1:47 pm

    Hiya,

    I am using active contour to segment cells accorss the cell cycle and I am having issues with mitosis. we image every 6 min but active contour often misses one or the 2 daughter cells at anaphase.

    I tried ticking “”watch entering objeects” which works well but have the issue that it now detects any debris or floating stuff as a cell.

    Is it possible to restrict the size of the “entering” objects?

    I tried playing with division sensitivity but it did not work (segemented my metaphase nucleus in 2).

     
    deborakeller0@gmail.com May 29, 2019 at 2:33 pm

    obviously you can clean up afterwards but it’d be good if it can be done before it generates additional areas..

    maybe it’s a feature request: restrict size of entering objects and min and/or max intensity

    Stephane Dallongeville June 3, 2019 at 11:38 am

    Hi Deborah,

    The problem is that Active Contour plugin already has many input parameters to say the least ! I think that it’s better to not increase it if possible to not make it more complex than it is already ^^ even more when we can just filter results afterward (this can be done automatically too, with a protocol). If the filtering is working in your case then it’s probably better going that way 🙂

    Best,

    – Stephane

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