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3-D3D3D coordinatesalignmentbatch processingCLEMcorrelationdeconvolutiondeformable registrationdenoisingdistance mapfeature detectionfeature matchingfilteringfluorescenceguiHSV visualisationimage processintensitymaskmeasurementmicroscopymorphologynon rigid registrationoperatorotsupluginpoint-spread functionprojectionregistrationroiscriptscriptingsegmentationsequencesmoothingspot countsurfacethresholdtoolboxtoolswarping