How to measure membrane thickness

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    Elena Deligianni October 29, 2020 at 4:08 pm

    Hello everybody,

    I am looking a way to measure the membrane thickness of cells stained with a membrane fluorescent marker.

    Any ideas?

    thank you

    Elena
    João Mamede October 30, 2020 at 12:11 am

    Fluorescence imaging should not have enough resolution to do so. Plasma membrane is around 10nm. Resolution of typical fluorescent microscopy is >300nm.
    Marion Louveaux November 10, 2020 at 2:09 pm

    Dear Elena Deglianni and Joao Mamede,

    We migrated on the image.sc forum and this forum is now closed. You can get more info here.

    Could you please re-post your question on the image forum and in the optionnal tags, write “icy”? This way the Icy team, but also the whole bioimage analysis community can help you and benefit from the answer.

    Many thanks in advance and see you soon on the image.sc forum!

    Best regards,

    Marion, for the Icy team

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